Job Description

The Development Sciences Biologics CMC Drug Substance Cell Line Development Group in South San Francisco, CA is seeking a Scientist I to join a talented and collaborative team developing CHO cell lines for GMP applications.

In this laboratory position, the candidate will perform hands-on molecular characterization studies in a well-supported and dynamic environment to help advance novel therapies to human clinical studies. The work will encompass molecular, cellular, and bioprocess development studies relating to the generation and characterization of optimized CHO cell lines for production of our pipeline candidates.

Key Responsibilities:

Perform CHO cell line development, including transfection, selection, subcloning, flow cytometry analysis, phenotypic and genotypic cell line screening.

Aseptically culture mammalian cells in suspension cultures across multiple scales and formats, from 96-well plates up to shake flasks.

Operate instruments like ViCell automated cell counter, Octet, Clone Select Imager, microscope, HPLC. Share responsibility for equipment maintenance and supplies.

Perform molecular biology techniques for CHO cell line development and characterization including DNA cloning and vector assembly, various types of PCR and RT-PCR, sequencing (Sanger, MinION and P2 NGS).

Collaborate with teammates to advance both routine and non-routine projects in which you will have to research, develop and implement new tools, technologies and approaches.

Display effective organization, verbal and written communication skills, and adherence to good documentation practices, including real time entry of lab work into electronic lab notebook.

Author detailed technical reports that support regulatory filings at all stages from IND to BLA.

Present updates at team meetings and collaborate closely with others in the cell line development group.