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Scientist - Stem Cells & Molecular Biology - NIH

West Bethesda, Maryland, US
Closing date
Dec 12, 2022

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Academic / Research
Conservation science
Salary Type
Employment Type
Full time
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(1) Prepare and conduct all aspects of mammalian cell culture and induced pluripotent stem cell culture, including media, reagent, buffer ordering and preparation, cell culture and propagation, and cryopreservation of cells.
(2) Implement induced pluripotent stem cell (iPSC) differentiation into a variety of cell types, including neuronal and glial cell types
(3) Design and conduct experiments using both fluorescence-imaging methodologies and bulk-phase molecular-biochemical techniques to address the mechanism of bacterial MinCDE dynamic molecular pattern self-organization on lipid bilayer.
(4) Explore the feasibility of developing cryo-EM sample preparation methodology for molecular imaging of protein pattern self-organization on lipid bilayer; learn cryo-EM technology.
(5) Explore the feasibility of developing cell-free reaction systems to study dynamic molecular pattern self-organization on the plasma membrane involved in eukaryotic membrane-based signaling systems.
(6) Coordinate the maintenance and performance improvement of the in-house custom-built TIRF-microscopes; train and mentor lab members on use of standard and custom-built TIRF-microscopes
(7) Perform basic laboratory experiments, including cell viability assessment, mycoplasma testing, performing assays, and confocal cell imaging experiments as well as performing relevant data analysis
(8) Perform biochemical assays including protein and organellar isolations, nucleic acid isolations, lipid isolations in iPSC-derived cell types.
(9) Organize the iPSC maintenance and differentiation training for new members of the lab
(10) Write manuscripts for publication in peer reviewed scientific journals.
(11) Provide routine activity updates, briefings, and presentations at group meetings
(12) Develop and present original research findings at professional and scientific conferences and workshops

(1) Ph.D. in Biology, Life Sciences, Molecular Neuroscience, or related discipline with strong record of productivity as evidenced by publication record
(2) Minimum of five (5) years of hands-on postdoctoral laboratory experience in bacterial cell biology, familiarity with eukaryotic cell biology and signaling systems, and experience with cell-free reconstituted systems
(3) Knowledge and mastery of mammalian tissue culture techniques including sterile technique, lentivirus production, molecular cloning, cellular transduction, transient transfection, and downstream processing assays.
(4) Experience with iPSC-derived neural cell culture
(5) Experience in molecular biology and biochemistry workflows including protein isolation, immunoprecipitation, and nucleic acid isolation
(6) Expertise in modern fluorescence microscopy image acquisition methodologies, image data processing/interpretation and quantitative image analysis methods and associated software along with general knowledge of modern high-performance microscope systems
(7) Experience with software including GraphPad Prism, SnapGene, Benchling, and image processing (FIJI/ImageJ)

Please Note: Vaccination against COVID-19 is a requirement for all job opportunities offered through Kelly Government Solutions, in accordance with the Executive Order on Ensuring Adequate COVID Safety Protocols for Federal Contractors.
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