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Senior Research Assistant (Islet Cell & Regenerative Biology)

Employer
Joslin Diabetes Center
Location
Boston, Massachusetts, US
Salary
Competitive
Closing date
Dec 27, 2022

View more

Sector
Academic / Research
Field
Conservation science
Discipline
Biology
Salary Type
Salary
Employment Type
Fellowship
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Under general supervision, the Senior Research Assistant will work on a project about beta cell aging.

Specifically, focusing on the insulin producing beta cells with the intention of gaining more insight into the effects of cellular aging upon the development, treatment and prevention of diabetes.

The project includes using molecular biology techniques, flow cytometry, histology, tissue culture, assays and animal work.

As a health care organization, we have the responsibility to do everything in our power to care for and protect our patients, our colleagues and our communities. We require that all staff be vaccinated against influenza (flu) and COVID-19 as a condition of employment.

Responsibilities

Data analysis using Microsoft Excel, Adobe Photoshop, Image J, Prism, among others.
Maintain a comprehensive notebook: electronic and on paper
Design and carry out experiments independently
Order and/or assist in preparation of laboratory reagents and solutions.
Assist in the onboarding and training of new lab members (students, fellows, staff, etc.)
Maintain inventories of laboratory supplies.
Arrange for equipment repair or maintenance.
Some of the techniques that will be used are:Monitoring of glucose metabolism such as: glucose and insulin tolerance tests on animals and glucose stimulated insulin secretion (GSIS) from isolated pancreatic islets.
Cell Culture: isolated pancreatic islets, various cell lines
Assays: glucose stimulated insulin secretion (GSIS), insulin content, viability, ELISA
Histology: tissue prep, staining, microscopy, sectioning, imaging and image analysis.
Flow cytometry, preparation of cells- dispersion, staining, sorting.
Animal work: Maintenance of mouse colonies, breeding and genotyping.

Other duties as assigned.

Qualifications

Bachelor's degree in Biological Sciences, Biochemistry, Chemistry, or a related discipline. Master's degree preferred.
1-3 years related work experience.
Previous undergraduate or post-graduate lab experience, particularly with mammalian cell culture and handling/screening of small animals will be preferred.
Willing to work with animals and human tissues
Basic knowledge of Microsoft Office Suite and ability to navigate at a basic level within various web-based applications.
Excellent data and notebook management (including use of electronic laboratory notebook)
Familiarity with basic laboratory instruments and equipment.
Ability to follow general instructions and procedures, as provided.
Strong organizational and communication skills (communicate clearly and effectively via verbal and written instruction).
Ability to prioritize responsibilities - through completion.
Ability to work independently and with others in the lab - to interact respectfully with other employees, professional staff and/or external contacts.

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Joslin is an Equal Opportunity Employer of Minority/Female/Individuals with Disabilities/Protected Veterans. Joslin values diversity and inclusion, and we encourage diversity applicants.

Joslin Diabetes Center is an Equal Opportunity Employer and it is the policy of Joslin to prohibit discrimination of any type and to afford equal employment opportunities to employees, and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, genetic information, marital/parental status, disability status, protected veteran status or any other status protected by law.

Joslin Diabetes Center is home to world-renowned scientists and researchers who are pushing to understand what causes diabetes - and to develop a cure for it.
Through clinical research in epidemiology, physiology, pathophysiology, health services, education, outcomes, and mental health, as well as an array of clinical trials, we are making great improvements in diabetes care.

Under general supervision, the Senior Research Assistant will work on a project about beta cell aging.

Specifically, focusing on the insulin producing beta cells with the intention of gaining more insight into the effects of cellular aging upon the development, treatment and prevention of diabetes.

The project includes using molecular biology techniques, flow cytometry, histology, tissue culture, assays and animal work.

Responsibilities:

Data analysis using Microsoft Excel, Adobe Photoshop, Image J, Prism, among others.
Maintain a comprehensive notebook: electronic and on paper
Design and carry out experiments independently
Order and/or assist in preparation of laboratory reagents and solutions.
Assist in the onboarding and training of new lab members (students, fellows, staff, etc.)
Maintain inventories of laboratory supplies.
Arrange for equipment repair or maintenance.
Some of the techniques that will be used are:Monitoring of glucose metabolism such as: glucose and insulin tolerance tests on animals and glucose stimulated insulin secretion (GSIS) from isolated pancreatic islets.
Cell Culture: isolated pancreatic islets, various cell lines
Assays: glucose stimulated insulin secretion (GSIS), insulin content, viability, ELISA
Histology: tissue prep, staining, microscopy, sectioning, imaging and image analysis.
Flow cytometry, preparation of cells- dispersion, staining, sorting.
Animal work: Maintenance of mouse colonies, breeding and genotyping.

Other duties as assigned.

Qualifications:

Bachelor's degree in Biological Sciences, Biochemistry, Chemistry, or a related discipline. Master's degree preferred.
1-3 years related work experience.
Previous undergraduate or post-graduate lab experience, particularly with mammalian cell culture and handling/screening of small animals will be preferred.
Willing to work with animals and human tissues
Basic knowledge of Microsoft Office Suite and ability to navigate at a basic level within various web-based applications.
Excellent data and notebook management (including use of electronic laboratory notebook)
Familiarity with basic laboratory instruments and equipment.
Ability to follow general instructions and procedures, as provided.
Strong organizational and communication skills (communicate clearly and effectively via verbal and written instruction).
Ability to prioritize responsibilities - through completion.
Ability to work independently and with others in the lab - to interact respectfully with other employees, professional staff and/or external contacts.

Joslin is an Equal Opportunity Employer of Minority/Female/Individuals with Disabilities/Protected Veterans. Joslin values diversity and inclusion, and we encourage diversity applicants.

Joslin Diabetes Center is an Equal Opportunity Employer and it is the policy of Joslin to prohibit discrimination of any type and to afford equal employment opportunities to employees, and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, genetic information, marital/parental status, disability status, protected veteran status or any other status protected by law.
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