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RA/SRA Cell Biology

Employer
Yoh, A Day & Zimmermann Company
Location
San Jose, California, US
Salary
Competitive
Closing date
Oct 8, 2022

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Sector
Academic / Research
Field
Conservation science
Discipline
Biology
Salary Type
Salary
Employment Type
Full time
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Yoh Life Sciences is seeking a Research Associate / Senior Research Associate, Cell Biology to support our clinical stage Immuno-Oncology client in the San Francisco Bay Area.

The Research Associate/Senior Research Associate will be responsible for validating novel oncology targets from the client's target discovery platform. Primary responsibilities involve identifying cancer cell lines expressing the selected target by qPCR and confirmation of protein expression.
Pay Rate: Depending on Experience
Industry: Immuno-Oncology
Location: San Francisco Bay Area
Type: Direct Hire

Responsibilities
Identifies cancer cell lines that express the selected cell surface target. This involves extracting mRNA from cancer cell lines and converting to cDNA, running qPCR experiments, and analyzing data. Rank cancer cell lines based on expression data.
Confirms protein expression on target-positive cell lines by flow cytometry or Western blot.
Uses Crispr-Cas9 editing for gene knock-out in cancer cell lines.
Maintains cancer cell lines using standard cell culture techniques.
Develops and validates primers for qPCR. Runs quality control on primers using multiple validation parameters.
Thoroughly and accurately documents all experiments using electronic laboratory notebook
Designs and executes experimental plans with guidance or minimal supervision; analyzes and records data, establishes laboratory protocols, and contributes to summary reports. Presents data at lab meetings.
Actively contributes to research ideas, experimental design, and assay development.
Isolates PBMC's from whole blood, purifies T cells by immunomagnetic selection, and grows cells in culture.
Supports in-house CAR-T cell program. This includes lentivirus production, transduction, and growing T cells. Performs functional assays, including cytotoxicity assays and measuring cytokine secretion by ELISA

Qualifications
BS/MS/PhD degree with 4-5 years of experience in molecular biology, immunology, biochemistry, cell biology, or related discipline.
Experience with qPCR
Experience designing and validating qPCR primers is strongly preferred.
Experience with cell culture techniques
Experience with standard immunology techniques (Flow cytometry, ELISA, WB)
Experience performing transient and stable transfections is optional.
Experience with CAR-T cells is optional.

Yoh, a Day & Zimmermann company, is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

Visit https://www.yoh.com/applicants-with-disabilities to contact us if you are an individual with a disability and require accommodation in the application process.
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