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E Talent Network
Rockville, Maryland, US
Closing date
Oct 19, 2022

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Academic / Research
Conservation science
Salary Type
Employment Type
Full time
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Job DescriptionOverall Position Summary and Objectives The contractor will independently provide support services to satisfy the overall operational objectives of the client.

Minimum Education

Resume Max Pages

Certifications & Licenses
  • N/A
Skills (Ranked By Priority)
  • Effective written and verbal communications skills
  • Enthusiasm for learning new techniques and concepts -Demonstrable evidence of resourcefulness and knowing how to troubleshoot basic technical problems with assays

  • Software
    • Gateway system
    Field of Study
    • Molecular Biology

    • Work products and documents related to conducting studies to extract DNA from endocrine-related mood disorder bloods; maintain DNA sample database; maintain inventory and archive of human tissue samples - Daily
    • Work products and documents related to processing human tissue samples for distribution to and collection from outside labs; send out samples for analysis; receive samples from outside labs; re-integrate samples back into BEB database and archive. - Daily
    • Work products and documents related to preparing blood samples to perform cell culture techniques for establishing patient cell lines and DNA re-extraction; track mood rating - Daily

    Designs and performs original molecular and cellular research.
    • Design molecular or cellular laboratory experiments, including molecular cloning, in vitro transcription, DNA/RNA analysis, protein expression in microbial systems, and protein purification and plasmid engineering.
    • Perform polymerase chain reaction (PCR) for cloning and amplification of DNA libraries for sequencing
    • Plasmid cloning and subclonining (primarily using the Gateway system)

    Evaluates, tests and implements new processes, methodologies and tools for complex molecular and cellular testing and research.
    • Performance of low and multiplex immunoaffinity assays including immunoblot, dot blot, immunoprecipitation, ELISA, cell-based assays, luciferase immunoprecipitation sequencing, and phage displays immunoprecipitation sequencing

    Documents research findings and maintains accurate logs and records of all experimental activities and outcomes.
    • Maintain precise and complete records of all experimental activities, to include methodologies, instrumentation and findings for futrure reference.
    • Maintain shared digital laboratory notebook. Maintain stocks of reagents and oversee/schedule routine equipment maintenance

    Provides subject matter expertise to senior scientists and program executives.
    • Immunohistochemistry of tissues and cells

    Prepares results of experimental findings for presentation and publication.
    • Storing, expanding, and maintaining mammalian cells line cultures for transfection
    • DNA plasmid preparation and gel electrophoresis
    • DNA/RNA extraction, purification, and preparation for send out sequencing
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