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E Talent Network
Rockville, Maryland, US
Closing date
Oct 19, 2022

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Academic / Research
Conservation science
Salary Type
Employment Type
Full time
Job DescriptionOverall Position Summary and Objectives The contractor will independently provide support services to satisfy the overall operational objectives of the client.

Minimum Education

Resume Max Pages

Certifications & Licenses
  • N/A
Skills (Ranked By Priority)
  • Effective written and verbal communications skills
  • Enthusiasm for learning new techniques and concepts -Demonstrable evidence of resourcefulness and knowing how to troubleshoot basic technical problems with assays

  • Software
    • Gateway system
    Field of Study
    • Molecular Biology

    • Work products and documents related to conducting studies to extract DNA from endocrine-related mood disorder bloods; maintain DNA sample database; maintain inventory and archive of human tissue samples - Daily
    • Work products and documents related to processing human tissue samples for distribution to and collection from outside labs; send out samples for analysis; receive samples from outside labs; re-integrate samples back into BEB database and archive. - Daily
    • Work products and documents related to preparing blood samples to perform cell culture techniques for establishing patient cell lines and DNA re-extraction; track mood rating - Daily

    Designs and performs original molecular and cellular research.
    • Design molecular or cellular laboratory experiments, including molecular cloning, in vitro transcription, DNA/RNA analysis, protein expression in microbial systems, and protein purification and plasmid engineering.
    • Perform polymerase chain reaction (PCR) for cloning and amplification of DNA libraries for sequencing
    • Plasmid cloning and subclonining (primarily using the Gateway system)

    Evaluates, tests and implements new processes, methodologies and tools for complex molecular and cellular testing and research.
    • Performance of low and multiplex immunoaffinity assays including immunoblot, dot blot, immunoprecipitation, ELISA, cell-based assays, luciferase immunoprecipitation sequencing, and phage displays immunoprecipitation sequencing

    Documents research findings and maintains accurate logs and records of all experimental activities and outcomes.
    • Maintain precise and complete records of all experimental activities, to include methodologies, instrumentation and findings for futrure reference.
    • Maintain shared digital laboratory notebook. Maintain stocks of reagents and oversee/schedule routine equipment maintenance

    Provides subject matter expertise to senior scientists and program executives.
    • Immunohistochemistry of tissues and cells

    Prepares results of experimental findings for presentation and publication.
    • Storing, expanding, and maintaining mammalian cells line cultures for transfection
    • DNA plasmid preparation and gel electrophoresis
    • DNA/RNA extraction, purification, and preparation for send out sequencing

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