Job Description: Job Description
When you join us, you'll be part of a smart, driven team that shares your passion for exploration and discovery. With revenues of rate billion and the largest investment in R&D in the industry, we give our people the resources and opportunities to make significant contributions to the world.
Be part of the genome engineering revolution. This scientist will develop tools and techniques to support genome engineering. This scientist will be expected to work in a highly collaborative team environment, supporting some projects with some supervision of a senior scientist and leading some projects independently. Projects may include, but not be limited to, cloning, developing gene editing strategies, and developing assays to monitor editing.
Scope of Need
The successful individual will directly interact with senior R&D scientists and project management to execute project work plans. The scientist will be responsible for supporting or executing these plans to meet agreed upon deliverables and timelines. These activities will require a high level of independence as well as the ability to work in a highly cooperative team.
This role will involve the following tasks:
• Mammalian cell culture
• DNA delivery and mammalian cell transfection
• Generation of Lentivirus
• Genomic editing in mammalian cell lines (TALN and CRISPR based editing, design and implementation)
• Flow cytometric analysis
• Molecular biology
o Plasmid subcloning, DNA preparations, sequencing, in vitro transcription of RNA, PCR
This individual will also be expected to support and participate in R&D and project management meetings.
Capabilities and Competencies
This position requires the following capabilities and competencies:
o Proven experience in cell biology
o mammalian cell culture
o Lipid-based cell transfection and electroporation
o Cell engineering (CRISPR or TALEN)
o DNA transposase
o Stable cell line generation
o Cell-based assays, such as FACS, immunocytochemistry, ELISA, and Western Blotting
o Experience in DNA transposition
o Sleeping beauty
o CRISPR-guided transposase
o Experience in molecular biology
o DNA cloning and sequencing
o DNA, RNA preparation
o PCR, qPCR assays
o Ability to:
o Observe deviations from the expected outcomes and trouble-shoot
o Succeed with a moderate level of supervision and guidance
o Participate in a strong teamwork orientation with demonstrable influence/interpersonal skills
o Maintain a detailed lab notebook and present your findings in a group setting
The Program Manager will interact with internal employees and management, as well as third-party providers, and customers.
The vast majority of working time will be spent in a laboratory environment. Cell work will take place in biosafety hoods in a tissue culture laboratory, working with various cell cultures. Time may be spent manipulating DNA constructs and proteins in a laboratory environment. Some time will be spent at the desk recording laboratory activities appropriately in notebooks.
Candidate must be capable of observing and adhering to lab safety standards and protocols.
This individual must be able to identify deviations from expected outcomes and participate in troubleshooting those deviations and suggesting alternatives.
Critical to success in this position will be the individual's ability to work independently on some projects and to provide support collaboratively on others while meeting project timelines.
Equally important will be the ability to work as part of a team in the laboratory (extended R&D team) as well as a broader cross-functional team (R&D, Project Management, Collaborator).
• A master's degree with 2+ years of experience in cell biology.
• PhD in cell biology
Indotronix is an Equal Opportunity Employer