Scientist I/II, Cellular and Molecular Biology
- Employer
- AbbVie
- Location
- Cambridge, Massachusetts, US
- Salary
- Competitive
- Closing date
- Aug 8, 2022
View more
- Sector
- Academic / Research
- Field
- Informatics / GIS
- Discipline
- Biology
- Salary Type
- Salary
- Employment Type
- Full time
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Transformational and Translational Immunology Discovery (TTID) at the AbbVie Cambridge Research Center is seeking a highly motivated and experienced scientist with immunology and molecular biology laboratory experience to join the CRISPR Center of Excellence, working collaboratively with TTID teams to advance the AbbVie immunology pipeline. This position requires passion, attention to detail, prior hands-on experience, and teamwork.
Responsibilities:
Independently performs cellular and molecular biology laboratory procedures, including immune cell culture, cellular functional assays, lentivirus generation, transfection, electroporation, and single cell cloning, nucleic acid and protein extraction, isolation, and purification, DNA sequencing, PCR, qRT-PCR, Western blot
Support CRISPR screening for target identification
Support target biology validation using genetic tools, including gene overexpression via ORF; CRISPR-based technologies; RNAi; mutagenesis; validation of genetic manipulations.
Effectively interpret results to peers and senior research staff
Work collaboratively on projects across multiple pipeline programs
Maintain accurate laboratory records and data.
Maintain expert proficiency in laboratory techniques and passionately learn additional workflows in support of new projects and adjacent teams
Basic Qualifications:
Bachelor's or Master's in molecular biology, immunology or other biological sciences with 5-7+ (BS)or 2-5+ (MS).
Skillful in cellular and molecular biology techniques, such as immune cell culture, lentivirus production, PCR, and gel electrophoresis; experience in genomic DNA isolation and validation of genomic edits, nucleofection using the Lonza 96 shuttle system
Experience with dendritic cell biology and a wide range of cell-based assays
Solid understanding of genetic tools such as CRISPR and RNAi-based technologies
Experienced in gene editing, RNAi, CRISPRa/i, or CRISPR KO strategies
Good communication, both speech and writing, to a variety of audiences
Ability to collaborate with peers and their teams
Preferred Experience:
Experience with high-throughput functional CRISPR screening; is a plus
Hands-on experience with immunoassays including FACS, ELISA, WB is preferred
Bioinformatics experience is a plus
Key AbbVie Competencies:
Builds strong relationships with peers and cross functionally with partners outside of the team to enable high performance
Learns fast, grasps the "essence" and can change course quickly where indicated
Raises the bar and is never satisfied with the status quo
Creates a learning environment, open to suggestions and experimentation for improvement
Embraces the ideas of others and nurtures innovation to make possibilities real
Position will be hired based on level of experience.PDN-ABV2202634
Responsibilities:
Independently performs cellular and molecular biology laboratory procedures, including immune cell culture, cellular functional assays, lentivirus generation, transfection, electroporation, and single cell cloning, nucleic acid and protein extraction, isolation, and purification, DNA sequencing, PCR, qRT-PCR, Western blot
Support CRISPR screening for target identification
Support target biology validation using genetic tools, including gene overexpression via ORF; CRISPR-based technologies; RNAi; mutagenesis; validation of genetic manipulations.
Effectively interpret results to peers and senior research staff
Work collaboratively on projects across multiple pipeline programs
Maintain accurate laboratory records and data.
Maintain expert proficiency in laboratory techniques and passionately learn additional workflows in support of new projects and adjacent teams
Basic Qualifications:
Bachelor's or Master's in molecular biology, immunology or other biological sciences with 5-7+ (BS)or 2-5+ (MS).
Skillful in cellular and molecular biology techniques, such as immune cell culture, lentivirus production, PCR, and gel electrophoresis; experience in genomic DNA isolation and validation of genomic edits, nucleofection using the Lonza 96 shuttle system
Experience with dendritic cell biology and a wide range of cell-based assays
Solid understanding of genetic tools such as CRISPR and RNAi-based technologies
Experienced in gene editing, RNAi, CRISPRa/i, or CRISPR KO strategies
Good communication, both speech and writing, to a variety of audiences
Ability to collaborate with peers and their teams
Preferred Experience:
Experience with high-throughput functional CRISPR screening; is a plus
Hands-on experience with immunoassays including FACS, ELISA, WB is preferred
Bioinformatics experience is a plus
Key AbbVie Competencies:
Builds strong relationships with peers and cross functionally with partners outside of the team to enable high performance
Learns fast, grasps the "essence" and can change course quickly where indicated
Raises the bar and is never satisfied with the status quo
Creates a learning environment, open to suggestions and experimentation for improvement
Embraces the ideas of others and nurtures innovation to make possibilities real
Position will be hired based on level of experience.PDN-ABV2202634
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