We are seeking a Research Assistant for Joslin's CRISPR Screen Core and Section for Islet Cell and Regenerative Biology. The laboratory is applying genome-wide CRISPR screen technique to investigate various questions in beta cell biology, including beta cell stress, function, allotransplantation tolerance, as well as human beta cell proliferation. The individual will be responsible for helping to design, execute, and analyze genome-wide CRISPR based knockout screen or overexpression screens in mammalian cells, as well as generate mutant cell lines for validation and follow-up studies, for projects within the lab and throughout Joslin. The individual will also assist in ongoing experiments involving beta cell protection, replication, and neogenesis. This position involves techniques in molecular biology, cell biology, and animal work.
Collaborate with investigators on the design and execution of CRISPR screens for questions related to Type 1 and Type 2 diabetes
Culture different mammalian cell types using sterile techniques
Generate lentiviral stocks for CRISPR-delivery
Perform various selection/sorting assays (Drug selection, flow cytometry, in vivo transplant, etc.)
Prepare DNA for high-throughput sequencing and analyze results
Clone plasmids to generate new constructs
Assist in troubleshooting procedures
Report on progress of work in laboratory meetings.
Maintain accurate, detailed, and up-to-date records of all procedures performed and results obtained
Place orders for supplies and help with general lab organization and maintenance
Train other lab members on techniques/procedures
Other duties as assigned
B.A. or B.S. in Biological Sciences, Biochemistry, Chemistry, or related discipline
Previous undergraduate or post-graduate lab experience
Familiarity with basic laboratory instruments and equipment
Experience with cell culture and molecular biology is preferred
Previous experience with CRISPR-based engineering and lentivirus is an advantage
Proficient in Microsoft Office Suite
Strong organizational, analytical, and communication skills
Ability to handle multiple projects simultaneously
Ability to work independently and with others
Joslin is an Equal Opportunity Employer of Minority/Female/Individuals with Disabilities/Protected Veterans. Joslin values diversity and inclusion, and we encourage diversity applicants.
Joslin Diabetes Center is an Equal Opportunity Employer and it is the policy of Joslin to prohibit discrimination of any type and to afford equal employment opportunities to employees, and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, genetic information, marital/parental status, disability status, protected veteran status or any other status protected by law.