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Scientist - Synthetic Biology

Replay Holdings LLC
Anaheim, California, US
Closing date
Jun 28, 2022

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Consultancy/Private Sector
Conservation science
Genetics, Biology
Salary Type
Employment Type
Full time
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We are seeking a full-time Scientist, who are highly experienced in Molecular Biology, DNA/gene synthesis, and/or emulsion-based chemistry. You will join our rapidly growing scientific team at Replay to design, engineer, and implement the next generation of virus-based gene therapies and synthetic biology platform technologies!

Replay is a start-up, VC-backed biotechnology company located in San Diego, CA focusing on developing gene therapy modalities and leveraging synthetic biology techniques to improve vector engineering and genome writing.

This position will give you the opportunity to take the lead in further developing and implementing virus genome engineering with multiple DNA assembly platforms, to deliver critical support to projects within the company. An ideal applicant has extensive experience in molecular biology or chemistry, a desire to develop synthetic biology technologies, and creative ideas about DNA assembly processes.

We are looking for a Scientist to:

· Implement multiple DNA assembly methods to construct gene therapy candidates

· Perform experimental planning, design independently and carry out experiments and data analysis

· Optimization of enzymatic reaction conditions

· Maintain organized records of experiments progress and scientific data

· Manage a small laboratory team

To meet these challenges, you will need to:

· Hold a PhD in a relevant area of Biological or Chemical Sciences and/or 5-10 years of relevant experience.

· Have experience in:

o Molecular biology techniques, especially in gene cloning and DNA assembly techniques, e.g. transformation-associated recombination (TAR), Gibson, Golden Gate, BioBrick, PCA

o Microbiology, including E. coli and/or yeast

o Gene synthesis chemistry and optimization, emulsion chemistry and/or microfluidics

· Be team-oriented, self-motivated, an effective communicator, and organized

Preferred Skills

Lab skills: Gene synthesis, emulsion-based chemistry, microfluidics, DNA synthesis, virus engineering, methods for high-molecular weight DNA, PCR, nucleic acid extraction, electrophoresis, including CHEF/FIGE, vector sequence design, cloning, primer design, tissue culture, virology (especially with herpesviruses), molecular genetics studies, CRISPR or other editing, NGS library preparation

Computer skills: DNA sequence programs (i.e., ApE, SnapGene, Geneious), electronic lab notebooks, some bioinformatic skills.

This position includes a full benefits package and a competitive compensation. Positions starting as soon as May 2022 in San Diego, CA

To apply please visit our job postings on LinkedIn

Or email your CV to
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