Research Scientist II, Molecular Biology RNA
We are seeking a highly motivated Research Scientist II with in-depth experience in Molecular Biology to join Drug Discovery Sciences (DDS) department at Takeda San Diego. This individual will perform various molecular biology assays including DNA/RNA manipulations and mammalian cell culture and support drug discovery projects. The successful applicant should have extensive bench research experience and enjoy a laboratory-focused role in a fast-paced, multidisciplinary team setting.
Manipulation of DNA (genomic DNA isolation/manipulation, recombinant plasmid generation/manipulation, lentivirus & adenovirus generation/transduction, gene editing by CRISPR, library prep for NGS) Manipulation of RNA (RNA isolation/manipulation, cDNA synthesis, design and transfection of siRNA/ASO, in vitro transcription, sample prep for NGS) Characterize protein expression and function using various molecular/cell biology and biochemical methods Generate, analyze and interpret data in appropriate scientific context, and present data in group meetings, project team meetings, and department meetings Regularly document experimental results in electronic notebooks
Technical/Functional (Line) Expertise
Demonstrates well-developed knowledge of related areas such as protein chemistry, cell biology and biophysics Strong understanding of the drug discovery process
Designs and directs research strategy and activities to meet program objectives; provide leadership, timelines and contingency plans
Decision-making and Autonomy
Independently manages workload and expectations Scientifically independent Scientific driver for research strategy that impacts group internally and outside area of function
Initiates and leads external interactions and collaborations Frequent contact with internal and external personnel at various management levels Collaborates with other departments, and disciplines, acting as scientific authority within discipline Serves as senior company / project representative at external conferences, or business meetings
EDUCATION, BEHAVIOURAL COMPETENCIES AND SKILLS:
Ph.D. in Cell/Molecular Biology, Biochemistry or related field with 1-3 years, MS with 4-6+, or BS with 6-8+ years of drug discovery experience in industry setting. Extensive experience with cloning, mammalian cell culture, transfection/transduction, siRNA/ASO design and transfection, western blot/HTRF/ELISA is required. Experience with high throughput assays with compound testing is strongly preferred. Knowledge on RNA splicing and protein translation is strongly desired. Must demonstrate the ability to design, execute experiments and troubleshoot independently. Strong communication skills and ability to work collaboratively in a fast-paced, multidisciplinary team setting are required.
In accordance with the CO Equal Pay Act, Colorado Applicants Are Not Permitted to Apply.
Takeda is proud in its commitment to creating a diverse workforce and providing equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, gender expression, parental status, national origin, age, disability, citizenship status, genetic information or characteristics, marital status, status as a Vietnam era veteran, special disabled veteran, or other protected veteran in accordance with applicable federal, state and local laws, and any other characteristic protected by law.
San Diego, CA