Please note this is a 5 month contract position.
This position in Gene Therapy and Gene Delivery Platforms group of Biotherapeutics offers an exciting opportunity to help develop novel Gene Therapies for unmet medical needs. We are investigating the use of Adeno-Associated Virus (AAV) vectors to deliver therapeutic proteins to patients for disease prevention as well as treatment of retinal, neuro, and infectious diseases.
We are seeking a highly motivated scientist to join a group that is engineering new AAV therapeutic vectors for lead indications and for evaluating advanced Gene Therapy technologies. These technologies include development of a scalable AAV manufacturing platform in human cells and improving AAV potency and tropism for relevant tissues and cell types.
The job requires a molecular biologist with experience cloning and purifying plasmids. Key characteristics include but are not limited to: ability to handle tight timelines and delivery of quality data; highly-organized and capable of handling multiple projects simultaneously; capable of carrying out established procedures and protocols with minimal direction.
Assist efforts to construct therapeutic plasmids and viral vectors using molecular cloning methods designed to maintain viral repeat regions
Purify plasmids by Qiagen anion-exchange chromatography columns
Perform restriction mapping and submit DNA sequencing samples to QC final plasmids
Organize and help maintain the plasmid inventory Maintain an electronic lab notebook (ELN) to document results
Contribute to a collaborative, creative, and rigorous culture of scientific discovery
Requirements Experience and Skills:
B.S. with at least 2 years or A.S. with 3-5 years of relevant post-graduate laboratory experience in the pharmaceutical industry or academic environment
Experience constructing plasmids using traditional restriction/ligation or isothermal assembly (In-Fusion/HiFi/Gibson) methods
Experience purifying plasmid DNA by Qiagen or similar anion exchange chromatography columns
Ability to follow established protocols with minimal guidance
Experience performing plasmid QC analyses, including diagnostic restriction digests and Sanger sequencing
A general understanding of virology and cell biology vectors
Cross-functional team experience in an industry environment
Experience with documentation and reporting of results using electronic lab notebooks is preferred
Applicant must provide their phone number. Job Reference #A1751.