This job has expired

Biologist

Employer
Medical Science & Computing
Location
Bethesda, Maryland, US
Salary
Competitive
Closing date
May 26, 2022

View more

Sector
Consultancy/Private Sector
Field
Conservation science
Discipline
Other
Salary Type
Salary
Employment Type
Full time
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Overview

We are currently searching for a Biologist to provide support to the National Institutes of Health (NIH). This opportunity is a full-time position with MSC, and it is on-site in Bethesda, MD.

Duties & Responsibilities
  • Prepare and/or isolate primary cells using fluorescent-activated or magnetic-activated cell sorting from specimens collected from individuals, non-human primates and other small animals.
  • Execute cultures of primary cells and cell lines with impeccable aseptic techniques and adherence to safety protocols.
  • Conduct blood processing and prepare serum, plasma, and PBMCs for testing as needed.
  • Perform functional assays with minimal oversight according to Laboratory Standard Operating Procedures, including but not limited the following procedures: ELISA, ELISpot, plaque and focus reduction neutralization tests [PRNT, FRNT], luminescence-based assays, flow cytometry and immunostaining, immunoprecipitations, and other biomarker detection assays.
  • Perform molecular biology procedures including but not limited to primer design, DNA and RNA extraction and purification, PCR, RT-PCR, RACE, sample preparation fro next-generation sequencing, molecular cloning, Western blots, gel electrophoresis, transfections, bacterial transformations (in particular), re related to the amplification of immunoglobulin genes and production of monoclonal antibodies.
  • Use large liquid handling automation systems and perform high-throughput screenings (HTS) workflows using various formats, including 384-well plates, and platforms.
  • Provide professional expertise in developing, revising, optimizing and validating Laboratory Standard Operating Procedures and Protocols for tissue/cell cultures, functional assays and molecular biology techniques, HTS workflows, including but not limited to the techniques mentioned above.
  • Produce, purify, quantify, and validate stocks of in-house made reagents, including proteins, antibodies from EBV-transformed LCLs and transiently transfected cell lines, and viruses (BSL [1] 2). Produce, biotinylate and fluorescent tagging of test proteins (e.g., tetramerization of biotinylated proteins using fluorescent streptavidin).
  • Prepare culture media, reagents and solutions according to specifications.
  • Maintain cell cultures (e.g., MS40L, IL2t, B95-8 cells), and perform virus amplification, harvest, and titration.
  • Apply Good Laboratory Practice (GLP) principles to characterize test articles, analyze specimens, and maintain detailed records of assays and data.
  • Assist with laboratory management, including assistance with the initial setup of the laboratory and subsequent management: order supplies and equipment; maintain and troubleshoot equipment (includes automated liquid handling systems, multimode readers, microscopes, plate washers, thermal cycler, biosafety cabinets and PCR hoods); serve as the primary emergency point-of-contact for storage equipment failures; maintain supply stocks and sample inventories; receive, organize, and distribute specimens; document shipments; assist PI with regulatory documentation.
  • Contribute to manuscript writing, and preparation and delivery of posters and oral presentations of scientific work.
Requirements
  • PhD in immunology, biology, chemistry or a related field is preferred.
  • Minimum of a Master's degree in immunology, biology, chemistry or related field.
  • Minimum of six (6) years of laboratory experience performing the serological and molecular techniques detailed above, as well as experience working with viruses and tissue culture.
  • Familiarity with automated liquid handling systems and high-throughput assays (384-well plate-based) preferred.
  • Demonstrated ability in conducting high-quality experiments, including dexterity in pipetting and skillfulness in aseptic techniques.
  • Knowledge of Microsoft Office software (Word, Excel and PowerPoint), statistical and scientific graphing programs (e.g., GraphPad Prism), accurate data entry in databases, and report generation.
  • Concise, clear and professional verbal and written communication.
  • Demonstrated skills in organization, problem solving, resourcefulness and flexibility.
  • Satisfactory US government background check and security clearance.
  • Foot travel within the NIH campus.
Due to our contractual requirements and federal orders, including an Executive Order from the White House and an emergency regulation from the Centers for Medicare & Medicaid Services (CMS), the position for which you are applying requires that you provide proof of your vaccination status. If you are unable to receive the COVID-19 vaccine for medical reasons or because of a sincerely held religious belief, you may request an exemption from the vaccination requirement which shall be reviewed after the submission of requested documentation. If an accommodation is granted, the conditions may include weekly testing and masking. All Guidehouse employees also agree to follow any additional health and safety mitigation policies that may be required in the workplace.

Company Description

Dovel Technologies and its Family of Companies (Medical Science & Computing and Ace Info Solutions) was acquired in October 2021.

Guidehouse is a leading global provider of consulting services to the public sector and commercial markets, with broad capabilities in management, technology, and risk consulting. By combining our public and private sector expertise, we help clients address their most complex challenges and navigate significant regulatory pressures focusing on transformational change, business resiliency, and technology-driven innovation. Across a range of advisory, consulting, outsourcing, and digital services, we create scalable, innovative solutions that help our clients outwit complexity and position them for future growth and success. The company has more than 12,000 professionals in over 50 locations globally. Guidehouse is a Veritas Capital portfolio company, led by seasoned professionals with proven and diverse expertise in traditional and emerging technologies, markets, and agenda-setting issues driving national and global economies.

Guidehouse is an Equal Employment Opportunity / Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to race, color, national origin, ancestry, citizenship status, military status, protected veteran status, religion, creed, physical or mental disability, medical condition, marital status, sex, sexual orientation, gender, gender identity or expression, age, genetic information, or any other basis protected by law, ordinance, or regulation.

Guidehouse will consider for employment qualified applicants with criminal histories in a manner consistent with the requirements of applicable law or ordinance, including the Fair Chance Ordinance of Los Angeles and San Francisco.

If you have visited our website for information about employment opportunities or to apply for a position, and you require accommodation, please contact Guidehouse Recruiting at 1-571-633-1711 or via email at RecruitingAccommodation@guidehouse.com. All information you provide will be kept confidential and will be used only to the extent required to provide needed reasonable accommodation.

Guidehouse does not accept unsolicited resumes through or from search firms or staffing agencies. All unsolicited resumes will be considered the property of Guidehouse, and Guidehouse will not be obligated to pay a placement fee.
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