Biological Scientist II
The University of Florida's College of Medicine's Department of Neurology is seeking a Molecular Biologist (Biological Scientist II) for the Laboratory of Neurogenetics and Neuroscience, which is physically based at the McKnight Brain Institute and in the UF Health Pathology Molecular Genetic Laboratory. Seeking candidates with an aptitude for population and pedigree-based molecular genetics and have experience with high-throughput sequencing, genotyping and Sanger sequencing. Applicants should also be familiar with bioinformatic methods for genome alignment, annotation and genetic variant analysis, although training opportunities will be offered.
Currently funded, IRB-approved, human research focuses on the molecular pathogenesis of neurologic and neurodegenerative disorders, and includes whole genome sequencing in amyotrophic lateral sclerosis, dementia and Parkinson's syndromes. Professor Farrer's team has previously authored articles for American Journal of Human Genetics, Nature Genetics, Science and specialty neurology journals, and their work has been featured in televised documentaries and public radio. We are looking for a candidate to contribute and share in that success, to help predict and prevent neurologic disorders, by applying molecular genetic methods.
Duties to include:
Most tasks are related to Illumina high-throughput genome sequencing, including prior DNA isolation from human tissues, sample plating, nucleic acid quantification, and genomic library preparation. Conduct and report quality control procedures at all stages of the workflow and perform routine bioinformatics processes. The position is to maximize sample throughput to minimize costs related to genetic sequencing. Work with Professor Farrer to review results and ensure research priorities are met.
ABI 3500 genetic analyses (Sanger sequencing, fragment size analyses and genotyping), Agena MASSarray SNP/CNV genotyping and real time qPCR for IRB-approved research projects in brain health and neurologic disease. Project-based research requires future planning and the ability to multitask. Laboratory work and related genetic analyses also require significant scientific/intellectual effort.
Genetic data analyses including quality control inspection, data cleaning, genotype and phenotype database integration, and secondary analyses that may include proprietary and public data sources, and requires familiarity with some genetic and statistical software packages.
To ensure all procedures and documents are maintained as required as part of good laboratory practice, including quality control and quality assurance. Participate in design and maintenance of laboratory safety programs and protocols. Help train lab personnel in all laboratory safety techniques, standard laboratory practice, genomic protocols and procedures including HIPAA compliance and relevant security/safety issues. Responsible for staying informed of current research findings, changes/enhancements in research studies and standards, technological advances, instrumentation, and software by reading scientific literature pertinent to the research area, conferring with vendors and attending professional conferences at which they may represent the university in a technical capacity.
Gainesville is a vibrant city in Alachua County and serves as the cultural, educational and commercial center for the North Central Florida region. The city is known for its nature parks, sporting events, museums, mild winters and warm summers. Being centrally located makes Gainesville easily situated for day trips to beaches along the Atlantic Ocean and Gulf of Mexico, and to theme parks including Disney World, and other cities including Orlando and Tampa. Learn more about Gainesville has to offer at Visit Gainesville.
$50,000 to $59,0000 annually; commensurate with education and experience.
Bachelor's degree in biology or a closely related field and three years of relevant experience; or a Master's degree in biology or a closely related field and one year of relevant experience; Doctoral degree in biology or a closely related field.
Graduate degree in molecular biology, biochemistry, genetics, or a related field.
5 or more years of experience with genetic and/or molecular biology techniques in a research or clinical laboratory.
Theoretical and applied practical knowledge of human genetics, genomics and molecular biology is necessary to perform DNA and RNA extractions, polymerase chain reaction (PCR), real-time quantitative PCR, genomic library preparation and quality control, Sanger and high-throughput sequencing data collection and associated analyses.
Experience in computational bioinformatics and statistical analyses for genome data. Familiarity in working in a UNIX environment, executing command line and genomics software packages (e.g. GATK, VCFtools, PLINK, R archive). Working knowledge of a computing languages is a plus (Python, R, C++).
Ability to multitask and work independently to troubleshoot problems, to collect, analyze, synthesize and present data.
Excellent teamwork, coordination, and organizational skills.
Excellent written, oral and interpersonal communication skills.
Special Instructions to Applicants:
In order to be considered, you must upload your cover letter and resume. Please include 3 to 5 professional references within the application.
The University of Florida is committed to non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, gender identity and expression, marital status, national origin, political opinions or affiliations, genetic information and veteran status in all aspects of employment including recruitment, hiring, promotions, transfers, discipline, terminations, wage and salary administration, benefits, and training.
This is a time limited position.
Application must be submitted by 11:55 p.m. (ET) of the posting end date.
Health Assessment Required: Yes