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Molecular Biology, Sr. Associate Scientist / Scientist

Employer
TrueBinding
Location
Menlo Park, California, US
Salary
Competitive
Closing date
Jan 26, 2022

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Sector
Consultancy/Private Sector
Field
Conservation science
Discipline
Biology
Salary Type
Salary
Employment Type
Full time
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About TrueBinding, Inc:

TrueBinding, Inc. is a preclinical and clinical stage biotech company developing first-in-class therapeutic antibody drugs for applications to treat cancer, metabolic diseases, immuno-fibrosis, autoimmune and neurodegeneration diseases, based on our proprietary TrueBinding platform. In our state-of-the-art laboratories, we are seeking innovative and disruptive therapies for highly unmet medical needs.

Position Overview:

TrueBinding Inc. is seeking a highly motivated Senior Associate Scientist or Scientist to join our Upstream Process Development (USP) team. The key responsibility in this role is lead projects for: 1) bulk production of recombinant proteins & antibodies in E. Coli , and/or stable or transient mammalian expression platforms; 2) molecular biology tasks to construct expression plasmids including lentivirus stocks. The ideal candidate is expected to have solid hands-on experience with molecular biology skills and recombinant protein / antibody production, and protein purification.

Responsibilities
  • Molecular cloning to construct plasmids for stable expression in mammalian cells; scale-up production of plasmid of different scales (Miniprep to Gigaprep);
  • Bulk production of proteins of interest;
  • Construct and generate Lentivirus stocks;
  • Manage the plasmid quality control, and maintain the plasmid inventory;
  • Closely collaborate with scientists in antibody engineering, protein purification, analytic, and in vivo pharmacology teams, to ensure timely delivery of the antibody with desired quality attributes;
  • Contribute to antibody production from stable cell lines, and to antibody titer determination;
  • Maintain accurate records and documentation, analyze, summarize, interpret, and communicate data internally;


Qualifications
  • BS or MS or recent Ph. D in Cell Biology, Biochemistry, Molecular Biology, and other related disciplines with 5+ (BS) or 3+ (MS) years of relevant experience;
  • Hands-on experience with routine molecular biology skills: primer design, PCR, molecular cloning, DNA sequencing result analysis, bacterial transformation, plasmid DNA extraction;
  • Knowledge and hands-on experience with Lentivirus highly desired;
  • Hands-on experience with protein chromatograph purification highly desired, experience with AKTA is a plus
  • Knowledge of common protein analytical methods and characterization is a plus;
  • Capable of organizing and present data internally, maintaining clear, accurate and complete documentation of experimental data and procedure;
  • Ability to work independently and as part of a team, self-motivation, adaptability, and a positive attitude;
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