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Scientist I, Molecular Biology

San Diego County, California, US
Closing date
Jan 26, 2022

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Academic / Research
Conservation science
Salary Type
Employment Type
Full time
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This position is responsible for therapeutic antibody screening from single B cell sorting and phage display libraries, cloning and expressing antigens, antibodies and their variants for our antibody discovery screening programs. Additional responsibilities include sequence analysis as it relates to antibody affinity maturation and target validation.

Essential Functions

· Design and implementation of optimized antibody screening strategies from human and mouse single B cells

· Construction of natural and synthetic antibody libraries for mammalian cell and phage display

· Design and implementation of phage display and mammalian cell display selection strategies

· Design, creation and implementation of cloning strategies using different cloning methods, PCR techniques and primer design

· Design, creation and construction of vectors for AnaptysBio libraries

· Design, optimization and implementation of RT-PCR from single cells and tissues

· Analyze and mine DNA sequences from Sanger and NGS

· Analyze sequence activity relationships to enable affinity maturation of antibodies

· Analyze RNA seq data for target validation

· Perform DNA mini-, midi-, maxi-prepping procedures

· Maintain and archive DNA clones, and bacterial glycerol stocks

· Organize, summarize and present data and results at department and project team meetings

· Assist/mentor junior, and new laboratory personnel

· Develop an expanding technical and theoretical knowledge base in molecular biology with a growing understanding of the scientific literature in relevant areas of research

· Maintain a detailed and well organized record of experiments in an electronic notebook

· Maintain familiarity with literature to propose and implement improved procedures and protocols, and to aid in problem solving

· May prepare technical reports, summaries and detailed protocols, and contribute to journal articles and company reports as requested

· Follow and adhere to SOPs and laboratory safety regulations


Education & Experience:
  • Theoretical knowledge typically achieved through post-graduate college experience, a PhD in Molecular Biology is preferred
  • 3+ years molecular biology experience.

Knowledge Requirements:

· Practical knowledge of phage display

· Extensive experience with protein expression and display vectors (promoter, transcriptional signals, etc.) and have the ability to analyze DNA and protein sequences

· Proficiency working with DNA analysis software programs such as Sequencher, DNAStar or their equivalent, and with Microsoft Office (Word, Excel, Powerpoint)

· Ability to recognize when it is appropriate and necessary to modify a standard procedure or protocol

· Consistent success in independently interpreting, and summarizing results, and in proposing next steps

Personal Competencies:

· Ability to communicate clearly, and to report and present experimental results and analysis to company colleagues

· Effective interpersonal skills, including excellent written and oral communication skills

· Ability to multi-task, organize multiple assignments, and work on several projects simultaneously while adhering to deadlines

· Ability to work effectively as a team player in a complex, changing environment

· Self-motivated, independent, and results-oriented

· Team player, intensely committed to success and getting the job done well in a highly dynamic environment

· Ability to work independently while also willing to follow protocols

· Strong analytical and organizational skills, with high attention to details

· Creates networks with key decision makers to effect change within the organization

Physical Demands

The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

While performing the duties of this job, the employee is regularly required to sit and talk or hear. The employee frequently is required to use hands to finger, handle, or feel. The employee is occasionally required to stand, walk, and reach with hands and arms. The employee must occasionally lift and/or move up to 10 pounds. Specific vision abilities required by this job include close vision and ability to adjust focus in order to read.

Work Environment

The work environment characteristics are representative of those an employee encounters while performing the essential functions of this job, typically in an open office environment. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

The noise level in the work environment is usually moderate.

This position requires working with biological and/or chemical hazards

All qualified applicants will receive consideration for employment without regard to race, color, sex, sexual orientation, gender identity, religion, national origin, disability, veteran status, age, marital status, pregnancy, genetic information, or other legally protected status.
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