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Biologist (Aseptic Technique and Mammalian Cell Culture)

GAP Solutions, Inc
Gaithersburg, Maryland, US
Closing date
Dec 4, 2021

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Academic / Research, Consultancy/Private Sector
Conservation science
Salary Type
Employment Type
Full time
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Position Objective: Provide services as a Biologist in support of the overall functions of the Vaccine Production Program (VPP), Vaccine Research Center (VRC) within the National Institute of Allergy and Infectious Diseases (NIAID). Duties and Responsibilities: * Support the Cell Line Development team's production of reagent-grade antibodies and other recombinant proteins * Culture, transfection, and selection of mammalian cells * Transient protein production * Stable pool generation and evaluation in fed-batch production * Support the Cell Line Development team's plasmid DNA supply system * Plasmid cloning and scale-up by PCR, restriction digest, and bacterial culture * Maintenance of plasmid database and coordination of receipt, ordering, and requests for plasmids * Generation of plasmid maps and confirmation of sequences * Provide general laboratory support of the Cell Line Development team * Preparation of medium, feeds, and reagents * Laboratory cleaning and biohazard waste removal * Maintenance of laboratory instrumentation and ordering of relevant supplies * Writing and reviewing standard operating procedures Basic Qualifications: * B.S. Biological Sciences with a minimum of 2 years of relevant work experience or M.S. in Biological Sciences with independent research experience in an academic or industrial setting. Advanced degree holders will not be considered. * Hands-on experience with aseptic technique and mammalian cell culture is required. * Familiarity with molecular cloning methods, PCR, and agarose gel electrophoresis as well as supporting software for generation and editing of plasmid maps is required. * Experience with inventory management and organization is required. * Experience in writing protocols and preparing data presentations is required. Minimum Qualifications: * Ability to work independently to execute defined experiments and troubleshoot issues as needed to meet project timelines. * Excellent skills in oral and written communications, computer competency, document control and attention to detail is required. * Detail-oriented and possess strong organizational skills with the ability to prioritize multiple tasks and projects. * Ability to effectively communicate with other team members in oral and written forms. * This job description is not designed to cover or contain a comprehensive listing of activities, duties or responsibilities that are required by this position. To perform this job successfully, an individual must be able to perform each essential duty satisfactorily. The requirements listed above are representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. GAP Solutions provides reasonable accommodations to qualified individuals with disabilities. If you need an accommodation to apply for a job, email us at You will need to reference the requisition number of the position in which you are interested. Your message will be routed to the appropriate recruiter who will assist you. Please note, this email address is only to be used for those individuals who need an accommodation to apply for a job. Emails for any other reason or those that do not include a requisition number will not be returned. GAP Solutions is an Equal Opportunity/Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to their race, ethnicity, ancestry, color, sex, religion, creed, age, national origin, citizenship status, disability, medical condition, military and veteran status, marital status, sexual orientation or perceived sexual orientation, gender, gender identity, and gender expression, familial status, political affiliation, genetic information, or any other legally protected status or characteristics. This position is contingent upon contract award.

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