Title: Senior Research Associate, Molecular Biology
Location: Cambridge MA
Duration: 6 months right to hire
We are seeking an experienced and highly motivated Research Associate/Senior Research Associate specializing in human iPSC techniques to join our discovery biology team.
The successful candidate will support the needs of the early target discovery team and platform development efforts by establishing protocols and generating various types of cells (neurons, microglia, hepatocytes, etc) from human iPSC's while designing and executing assays to support target validation.
A strong background in molecular biology and cell biology techniques is essential, with the ability to perform experiments independently in a fast-paced environment are essential.Responsibilities
- Establish robust protocols to generate and scale the development of various specific cell types (hepatocytes, neuron, microglia, etc) from human pluripotent cells to model multiple human diseases
- In collaboration with the discovery team, develop and implement molecular and functional assays such as ER stress, lysosomal function, metabolic assays in iPSC derived cells.
- Design, execute, and interpret multiple experimental workflows including standard cell biology, imaging and stem cell biology methods such as qPCR, protein detection, ELISA and other relevant biochemical, molecular and cell biology methods.
- Proven expertise in developing human stem cell approaches, applications to unique biological problems, profiling, and assay development
- Deep knowledge of human pluripotent stem cell culture, characterization, QC, and differentiation (hepatocytes, neuron, microglia, etc)
- Proven hands-on experience with a variety cell-based and molecular biology readout technologies, such as RNAseq, FACS, flow cytometry, qPCR, immunostaining, high-content and confocal imaging
- Demonstrated ability to work in a dynamic environment as a team player with a strong work ethic, excellent communication, and time management skills
- Clearly communicate results and recommendations to colleagues, managers, and senior management, while providing technique-based advice on project decision-making
- Maintain diligent and organized records.
- BSc or MSc degree, or equivalent, in molecular biology, genomics, biology, or biomedical engineering with at least 1-2 years of relevant industry or academic research experience
- Hands-on practical experience in stem cell biology techniques along with advanced understanding of iPSC differentiation process
- An interest to train in and contribute to other areas of the target validation team
- Strong organization, communication, and interpersonal skills are required, with the ability to work independently
- and on a team