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SOAR - NIEHS - Animal Biologist Small Animal

Axle Informatics
North Bethesda, Maryland, US
Closing date
Oct 14, 2021

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Academic / Research
Conservation science
Salary Type
Employment Type
Full time
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SOAR - NIEHS - Animal Biologist Small AnimalOverall Position Summary and Objectives:The biologist will be responsible for day-to-day operation of the Laser Capture Microdissection core laboratory and will be skilled in making cryosections, basic histology stainings, immunohistochemical staining, immunofluorescence, and extraction of nucleic acids from LCM material. In addition, they will have basic knowledge of molecular biology and cell culture techniques. In addition to the responsibilities in the LCM core laboratory, they will assist ongoing research activities in the Molecular Pathology laboratory and these studies can include literature review, rodent studies, necropsy, and sample collection, preparing NGS sequencing libraries, and assist in experimental validation of data. Also, they'll help in mentoring trainees.Deliverables:Clean Equipment - DailyInspect Equipment - DailyMeet with lab members to present updates WeeklyWork Details:Studies the basic principles of animal life and the effects of varying environmental factors. Coordinate husbandry, animal care and enrichment activities to maintain a clean, safe and regulatory compliant animal research program Conducts research and performs experiments on the selection and breeding of animals. Update animal protocol through the submission of amendments and submission of new animal protocols to ensure necessary animal work for experiments Anticipate animal needs for lab research and colony maintenance; adjust the number of breeding pairs accordingly Maintains animal colonies. Handle, dissect and euthanize animals Maintain animal colony, including general husbandry, breeding, plug checks, weaning, tagging animals, and obtaining tail or ear snips for genotyping, and euthanasia Performs simple surgeries on animals. Handle, dissect and euthanize animals Prepares and analyzes animal tissues. Perform experiments with primary tissues and cells in culture, utilizing sterile techniques Harvest and collect animal embryos, tissues, and organs Perform a variety of specialized tissue fixation, embedding, sectioning and staining techniques Maintain and culture human and animal cancer cell lines for experiments Perform analysis of animal tissues; prepare frozen slices and paraffin sections, appropriately stained to demonstrate normal and abnormal structures Performs molecular biology techniques. Perform a variety of specialized tissue fixation, embedding, sectioning and staining techniques Perform genomic DNA preparation, genotyping and other analyses of animal tissues using DNA extraction and PCR Carry out molecular biological experiments, including extracting genomic DNA, performing PCR and gel electrophoresis, cloning DNA fragments, and preparing plasmid DNA, generating high quality RNA using in vitro transcription Perform standard molecular biology techniques, including, but not limited to, cloning, in situ hybridization and qPCR Maintain cell lines and perform basic cell culture assays, including, but not limited to, transfection and immunocytochemistry Performs immunohistochemistry on animal tissues. Perform light and confocal microscopy Perform apoptosis assays, qtr-PCR, Western blotting, immunohistochemistry, and molecular cloning techniques Work with staff to process tissue for immunohistochemistry Perform Western Blot, immunohistochemistry, and immunofluorescence Isolate and purify nucleic acid and proteins from tissue culture samples Perform molecular biology and cloning techniques, tissue histochemistry and immunohistochemistry Analyzes experimental data. Update and maintain laboratory notebooks and databases; collect, analyze, and interpret data Perform quantitative analysis; data analysis, figure preparation, and image acquisition; maintain complete and detailed records of all experiments Collect, organize, and record experimental data for publication and future reference by others Collect, review, research, and analyze large datasets; perform experiments, utilizing various statistical applications and methods; and interpret data, assemble data into figures, and enter data into a database Analyze and interpret scientific data from animal cell lines Collate, edit, and archive electronic and paper laboratory files, notebooks and databases Analyze and catalog imaging data from molecular biology and in vitro tissue culture assays Maintain records of experimental results; work with staff to analyze and interpret data; prepare summaries of laboratory findings Perform computer analysis and use computer software to prepare images Edits and writes research papers and protocols. Prepare abstracts and present posters Prepare reports and manuscripts based on experimental results Participate and collaborate in the preparation of scientific publications for peer reviewed journals based on the results Document information on procedures, modifications, and methods for input into final publication and presentations of findings at meetings Work with staff on the preparation of data and images for use in research presentations and publications Write and edit drafts for journal submission; read manuscript drafts of other laboratory members Compile, write and present scientific data in conferences; write articles Edit and write research papers and protocols Selects, devises, plans, and executes the most appropriate experiments for analysis and interpretation of data, for developing strategies, for pursuing significant observances, and for ensuring accuracy of data. Work with staff on the modification of laboratory protocols and experiments based on literature findings 5Search and review literature related to project; summarize and evaluate findings Document all laboratory procedures, experiments, and results; maintain and update laboratory notebooks and databases 4Develop, validate, perform, and interpret a variety of complex experimental techniques and procedures; work with staff to develop procedures addressing new animal study needs, logistics, modifications to standard operating procedures, and/or existing guidelines to resolve technical problems 1Collaborate with team members to determine, evaluate, and discuss the progress of projects; point out problems and suggest solutions to problems or augmentation of experimental approaches 3Participate in developing and performing experiments, organizing protocols, and performing technical troubleshooting Locate and review the literature pertinent to projects to identify the most applicable protocols and methods; perform experiments in accordance with protocols Manage oversight and progress of critical results in animal-based experiments including evaluating goal-specific phenotypes of animals; collaborate with Primary investigators and other experimental team members to determine, evaluate, and discuss the progress of projects 21, 2, 3, 4, 5 represents priority rankings, where 1 is highest priority and 5 is lowest priority of those rankedQualifications:Education: BachelorsSkills (Ranked By Priority)Laser Capture MicrodissectionCryosectioningHistology techniquesBasic Molecular biology skills1, 2, 3, 4, 5 represents priority rankings, where 1 is highest priority and 5 is lowest priority of those rankedSoftwareMS officeStatistical softwareImage analysisField of StudyMolecular BiologyGeneticsEnvironmental ScienceBenefits100% Medical Dental & Vision Coverage for EmployeesEducational Benefits for Career GrowthPaid Time Off (Including Holidays) Employee Referral Bonus Profit Sharing401K MatchingFlexible Spending Accounts:Healthcare (FSA)Parking Reimbursement Account (PRK)Dependent Care Assistant Program (DCAP)Transportation Reimbursement Account (TRN)The diversity of Axles employees is a tremendous asset. We are firmly committed to providing equal opportunity in all aspects of employment and will not tolerate any illegal discrimination or harassment-based age, race, gender, religion, national origin, disability, marital status, covered veteran status, sexual orientation, status with respect to public assistance, and other characteristics protected under state, federal, or local law and to deter those who aid, abet, or induce discrimination or coerce others to discriminate.Accessibility: If you need an accommodation as part of the employment process, please The above is meant to illustrate the general nature of work and level of effort being performed by individuals assigned to this position or job. This is not restricted as a complete list of all skills, responsibilities, duties, and/or assignments required. Individuals may be required to perform duties outside of their position, job or responsibilities as needed.
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