Computational Biologist

San Jose, California, US
Jul 24, 2021
Aug 02, 2021
Employment Type
Full time
Salary Type
Job Description
Cellecta, a small, privately-owned Mountain View, CA based biotechnology company providing tools and technology to life science laboratories, is looking for a skilled computational biologist to provide design, analysis, and development support for its product and service portfolio.

The company focuses on developing and marketing innovative tools for bulk, single cell and spatial RNA expression profiling, CRISPR/shRNA genetic screening, cell barcoding, and related functional genomics/transcriptomics NGS-based assays. For this role, we are seeking a proactive self-motivated individual to be in charge of the design work for custom pooled sgRNA/shRNA/barcode libraries, as well as the analysis of NGS data from CRISPR and RNAi genetic screens (including Perturb-seq), RNA expression profiling, spatial transcriptomics, etc. The role would also support R&D efforts in developing the product portfolio.

  • Advanced understanding of general genomics and molecular biology with a specific focus on multiplex PCR, NGS, library construction.
  • Practical experience with designing oligo-based reagents (e.g., sequencing/PCR primers, sgRNA, shRNA, etc.) for genomic analysis and NGS.
  • Experience deconvoluting, analyzing, interpreting, and reporting on NGS data derived from genetic screens, RNA profiling, single cell expression profiling (10x) and similar complex genomics/transcriptomics analysis. Direct experience managing NGS data pipeline from sequencing run to report would be a plus.
  • Demonstrated ability to develop programs and create routines using general-purpose programming languages such as C++, Python, Java, etc., to automate data processing, data analysis, and manage information.
  • Familiarity and ability to mine information from publicly available genome databases such as NCBI, Ensembl, HUGO, and Pfam, and use tools such as Reactome and PANTHER for basic data interpretation and pathway analysis. Experience with cancer biology and immunology is desirable.
  • Some background in developing and managing central databases and/or web-based portals and applications to enable user access to sequence information, design tools, and/or analysis reports is desirable.

Applications from principals only please. Cellecta is an Equal Opportunity Employer.
Company DescriptionCellecta Inc., a functional genomics solutions provider, focuses primarily on developing and implementing flexible and scalable broad-based screening and analysis approaches for drug target and biomarker discovery. Our high-throughput functional genetic screening portfolio includes:

* shRNA and CRISPR screening products and services, custom and off-the-shelf constructs and pooled libraries, knockout, knock-in and knockdown cell lines that facilitate genome-wide functional screening and the identification and validation of genes involved in critical biological and disease pathways.

* Our CloneTracker Barcode products enable tracking of cell populations and progeny with stable, genomically integrated, single, identifiable, sequenceable barcodes.

* With DriverMap Targeted RNA Expression Profiling products and services, Cellecta offers a quantitative, multiplexed approach that allows simultaneous profiling of all human protein-coding genes--or any subset thereof--in one reaction, to provide a reliable, accurate transcriptome profile with ultra-high sensitivity.