Scientist, iPSC Cell Therapy/CAR T, Exploratory Biology

Employer
J&J Family of Companies
Location
Spring House, Pennsylvania, US
Salary
Competitive
Posted
Jul 24, 2021
Closes
Aug 06, 2021
Ref
2279192515
Employment Type
Full time
Salary Type
Salary
Janssen Research & Development, L.L.C., a division of Johnson & Johnson's Family of Companies is recruiting for a Scientist, iPSC Cell Therapy/CAR T located in Spring House, Pennsylvania.
At the Janssen Pharmaceutical Companies of Johnson & Johnson, we are working to create a world without disease. Transforming lives by finding new and better ways to prevent, intercept, treat and cure disease inspires us. We bring together the best minds and pursue the most promising science. We are Janssen. We collaborate with the world for the health of everyone in it. Learn more at www.janssen.com and follow us @JanssenGlobal. Janssen Research & Development, LLC is part of the Janssen Pharmaceutical Companies.
General Description of the role :
We seek a highly motivated and independent Scientist to join our iPSC team in the Exploratory Biology group of Janssen Biotherapeutics. The Scientist, iPSC Cell Therapy/CAR-T will be responsible for design and execution of experiments enabling development of a robust platform to generate genetically engineered, iPSC-derived cell therapies for oncology and other indications. Key activities will include optimize differentiation methods, phenotypic and functional characterization of immune cells using in vitro and in-vivo methods to generate novel cell therapy products (CAR-T, CAR-NK, etc.) from iPSCs. The successful candidate will utilize a full suite of advanced technologies to develop best-in-class, off-the-shelf cell therapy drug candidates.
Primary responsibilities:
- Technical experience in immunology and oncology research with preferred experience in T/NK cell biology including T/NK cell functional assays
- Application of extensive knowledge of T/NK cell biology including activation pathways, signaling pathways and antigen-specific differentiation
- Independently design and execute experiments to optimize protocols for iPSC differentiation to therapeutic immune cells including T cells, NK cells, and other immune cell types
- Extensive phenotypic characterization of lymphoid cells using multicolor flow cytometry, mass cytometry and other state of the art methods
- Functional characterization of iPSC derived lymphocytes, T and NK cells assays such as proliferation, cytotoxicity, cytokine release, and ADCC assays
- Experience working with xCELLIgence, Incucyte, Isoplexis and Seahorse technologies are highly desirable
- Coordinating experiments with other scientists across functional groups, compile data and present results and stay current on relevant primary literature
- Effectively manage multiple projects while adhering to aggressive timelines
- Accurately record and document results in a timely fashion
- Foster a collaborative, creative, and rigorous culture of scientific discovery
Qualifications
- Ph.D. in immunology, immune-oncology or tumor immunology with at least 3 years of successful postdoctoral experience as demonstrated by strong publication record plus at least 3 years of experience in an industry setting or a M.S. with a minimum of 12 years of relevant work experience or B.S. with minimum of 14 years of relevant work experience in T/NK cell immunology
- Experience with in vitro technologies typically employed to assess lymphocyte functionality including but not limited to MSD, IncuCyte-based assays, multi-parameter flow cytometry for phenotypic characterization of T/NK cells, T/NK cell activation assays, T/NK cell proliferation analysis, imaging is required
- Immune cell biology expert able to apply skills, theoretical knowledge, and experience to provide scientific direction and approach to projects and challenges is preferred
- Strong working experience with NSG xenograft mice model for CAR-T/NK immunobiology and oncology, dose response related studies using bioluminescence imaging (BLI).
- Experience working with viral (Retro or Lenti-virus) and non-viral delivery methods, ELISA, qRT-PCR, cell-based potency assays
- Knowledge in gene editing technology is plus
- Self-motivated and focused with project management-oriented thinking; able to work on multiple projects in a matrix environment
- Strong communication skills for working with cross-functional teams as well for cross-departmental colleagues
Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability.
Primary Location
United States-Pennsylvania-Spring House-Welsh & McKean Roads
Organization
Janssen Research & Development, LLC (6084)
Job Function
R&D
Requisition ID
2105944022W