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Biologist

Employer
Global Channel Management, Inc.
Location
Indianapolis, Indiana, US
Salary
Competitive
Closing date
Jul 24, 2021

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Sector
Academic / Research, Consultancy/Private Sector
Field
Conservation science
Discipline
Other
Salary Type
Salary
Employment Type
Full time
Biologist needs 3+ years experience
Biologist requires:
B.S. in biology with experience working in an academic or industrial research laboratory
Hands-on experience and familiarity with contemporary techniques in cell and molecular biology research fields is a must.
Experience on generation of stable cell lines with various cancer cells is desired
Work experience in cancer biology related research is a big plus
Ready to take on new challenges and open to learning new skills

Biologist duties:
Work closely with research scientist in a cell and molecular laboratory and perform experiments daily Maintain, passage various adherent or suspension cancer cell lines, prepare cryopreservation frozen stock for long term storage.
Conduct and assist to conduct plasmid DNA transfection of cancer cell lines using lipid based reagents, electroporation, as well as viral based methods.
Conduct and assist to conduct experiments to select and generate sub-lines with stable exogeneous expression of gene of interest from cell transfection.
Perform cell based assays including cell proliferation assays, cell survival assays with cytotoxic compounds using multiple technology platforms
Perform biochemistry assays including western blotting, ELISA, multiplex ELISA
Perform molecular biology assays including DNA, RNA isolation from cell culture and tissues, RT-qPCR for gene expression analysis
Assist in other experiments and laboratory maintenance related activities
Maintain accurate laboratory records and data.
Do initial data analysis in Excel and other analysis software, prepare experiment results summary
Work with scientists from other teams in a collaborative environment

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