Biologist (3-5 years)

Employer
System One
Location
Indianapolis, Indiana, US
Salary
Competitive
Posted
Jul 20, 2021
Closes
Jul 31, 2021
Ref
2329457653
Discipline
Energy
Employment Type
Full time
Salary Type
Salary
Title: Biologist (3-5 years)
Location: Indianapolis, IN
Start date: ASAP

Responsibilities:
  • Work closely with research scientist in a cell and molecular laboratory and perform experiments daily
  • Maintain, passage various adherent or suspension cancer cell lines, prepare cryopreservation frozen stock for long term storage.
  • Conduct and assist to conduct plasmid DNA transfection of cancer cell lines using lipid based reagents, electroporation, as well as viral based methods.
  • Conduct and assist to conduct experiments to select and generate sub-lines with stable exogeneous expression of gene of interest from cell transfection.
  • Perform cell based assays including cell proliferation assays, cell survival assays with cytotoxic compounds using multiple technology platforms
  • Perform biochemistry assays including western blotting, ELISA, multiplex ELISA
  • Perform molecular biology assays including DNA, RNA isolation from cell culture and tissues, RT-qPCR for gene expression analysis
  • Assist in other experiments and laboratory maintenance related activities
  • Maintain accurate laboratory records and data
  • Do initial data analysis in Excel and other analysis software, prepare experiment results summary
  • Work with scientists from other teams in a collaborative environment
  • Identify and troubleshoot assay issues and work on improvements
  • Self-motivated, capable of multi-tasking and able to ensure focused and timely execution of workload
  • Pay attention to all detail parts of complex experiment protocols
  • Monitor or operate specialized equipment such as electrophoresis units, thermocyclers, cell imagers, phosphor-imagers

Requirements:
  • Bachelor's Degree in Biology with experience working in an academic or industrial research laboratory
  • Hands-on experience and familiarity with contemporary techniques in cell and molecular biology research fields is a must.
  • Experience on generation of stable cell lines with various cancer cells is desired
  • Work experience in cancer biology related research is a big plus
  • Ready to take on new challenges and open to learning new skills

Joule, a System One division is a leader in specialized workforce solutions and integrated services. With more than 6,000 employees and roughly 50 offices throughout the U.S. we provide scientific, clinical, engineering, energy, IT, legal and administrative staffing services. For more than 40 years, we have delivered workforce solutions and integrated services to clients nationally.

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