NIH-NIAID-DIR-Biologist

3 days left

Employer
Axle Informatics
Location
Bethesda, Maryland, US
Salary
Competitive
Posted
Jul 08, 2021
Closes
Aug 03, 2021
Ref
2343879372
Discipline
Other
Employment Type
Full time
Salary Type
Salary
Axle Informatics is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations around the globe. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).

Overview

Axle is seeking a highly ambitious, adaptable, structured, and detail-oriented Biologist to join our vibrant team at the National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), Division of Division of Intramural Research (DIR), Laboratory of Immunoregulation (LIR), Bethesda, MD.

Responsibilities
  • Provide support of lab activities related to the structural biology of viruses and vaccine particles and vaccine development, such as but not limited to: prokaryotic and eukaryotic cell culture techniques for the production and purification of influenza viruses and the production of recombinant protein antigens and antibodies by gradient centrifugation and column chromatography.
  • Perform cell culture techniques related to cell-based assays such as antibody-dependent cellular cytotoxicity (ADCC,) microneutralization, Median Tissue Culture Infectious Dose (TCID50) assays would be preferred
  • Perform general biochemical and immunological assays such as hemagglutination inhibition assay, SDS-PAGE, Western blot, and ELISA.
  • Perform and analyze immunological assays according to Laboratory Standard Operating Procedures, including but not limited to Western Blot, ELISA, ADCC, microneutralization, and (TCID50) assays, HAI and other inhibition assays.
  • Perform molecular biology/virology methods including but not limited to DNA extraction and purification, PCR, RT-qPCR, molecular cloning, gel electrophoresis, transfections, bacterial transformations, recombinant protein production/purification and microscopy.
  • Apply Good Laboratory Practice (GLP) principles to characterize test articles, analyze specimens, and maintain detailed records of assays and data.
  • Maintain cell cultures (e.g., Vero, MDCK cells), and perform virus amplification, harvest, and titration
  • Receive, organize, and distribute specimens, documenting shipments using specified electronic systems.
  • Apply Good Laboratory Practice (GLP) principles to characterize test articles, analyze specimens, and maintain detailed records of assays and data.
  • Maintain cell cultures (e.g., Vero, CRFK, Sf-9, CHO, RAW264.7, CaCo-2 cells, intestinal enteroids), and perform virus or VLP amplification, harvest, and titration.

Experience Required / Requirements
  • Bachelor's and/or Master's degree in biology or a related field.
  • Minimum of six years of laboratory experience performing the serological and molecular techniques detailed above, as well as experience working with viruses and tissue culture.
  • Knowledge of Microsoft Office software (Word, Excel and PowerPoint) and report generation.
  • Concise, clear and professional verbal and written communication.
  • Demonstrated skills in organization, problem solving, resourcefulness and flexibility.
  • Demonstrated ability in conducting high-quality experiments, including dexterity in pipetting.
  • Foot travel within the NIH campus

Benefits
  • 100% Medical, Dental & Vision premium coverage for Employees
  • Paid Time Off (Including Holidays)
  • Employee Referral Bonus
  • 401K Match
  • Tuition reimbursement and professional development opportunities
  • Flexible Spending Accounts:
  • Healthcare (FSA)
  • Parking Reimbursement Account (PRK)
  • Dependent Care Assistant Program (DCAP)
  • Transportation Reimbursement Account (TRN)


The diversity of Axle's employees is a tremendous asset. We are firmly committed to providing equal opportunity in all aspects of employment and will not tolerate any illegal discrimination or harassment-based age, race, gender, religion, national origin, disability, marital status, covered veteran status, sexual orientation, status with respect to public assistance, and other characteristics protected under state, federal, or local law and to deter those who aid, abet, or induce discrimination or coerce others to discriminate.

Accessibility: If you need an accommodation as part of the employment process please contact: 

Email: careers@axleinfo.com

Disclaimer: The above description is meant to illustrate the general nature of work and level of effort being performed by individual's assigned to this position or job description. This is not restricted as a complete list of all skills, responsibilities, duties, and/or assignments required. Individuals may be required to perform duties outside of their position, job description or responsibilities as needed.

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