Research Associate, Molecular Biology

3 days left

Employer
R&D Partners
Location
Campbell, California, US
Salary
Competitive
Posted
Jun 27, 2021
Closes
Aug 06, 2021
Ref
2282495168
Discipline
Biology
Employment Type
Full time
Salary Type
Salary
Position Summary:

A local Biotech company is currently seeking a creative and detail-oriented Research Associate with experience in molecular biology to support the internal pipeline technology program within the platform and discovery group. This role requires a core focus on the construction of new molecular plasmids, assay development supporting molecular gene editing, and generation of stable, clonal cell lines.. Hands-on experience with techniques in genome engineering and virus transduction methodologies are highly desirable.

The successful candidate will be both a strong lab-based individual contributor and a team player who works well with other scientists. The candidate will contribute to key discovery studies which will have a direct impact on the success of our therapeutic programs.

Responsibilities:
  • Perform molecular cloning to assemble novel plasmid DNA constructs.
  • Perform standard PCR and RT-qPCR assays in mammalian cells and cell lines.
  • Perform and optimize transfection and transduction for delivery of plasmids and viral vectors for stable and transient gene expression in cell lines.
  • Maintain cell lines through transfection and selection for the generation of transient and stable clones.


Duties:
  • Perform in vivo gene editing using CRISPR-associated (Cas) proteins.
  • Express recombinant proteins and characterize protein expression using standard assays (e.g., SDS-PAGE, western blot).
  • Accurately document experimental protocols, perform analysis and help with interpretation of data, and report key findings to senior management and research team.
  • Proficiency in working with various DNA analysis software programs such as Snapgene, Lasergene or their equivalent, and with Microsoft Office (Word, Excel, Powerpoint).


Requirements:
  • B.S. in molecular and cellular biology, biochemistry, immunology, or a related field. 2 years (or more) relevant laboratory-based experience in industry or academia.
  • Experience with molecular cloning, including plasmid DNA construction, transformation, and isolation, nucleic acid extraction, DNA mini- and maxi-prepping procedures and DNA sequence analysis.
  • Experience in gel-based assays (DNA gels, protein gels, western blot)
  • Experience in genomic DNA isolation and mRNA purification, and RT-qPCR
  • Experience in standard tissue culture techniques and maintaining cell lines.


Preferred Qualifications:
  • Expertise in vector construction techniques such as PCR, Gibson assembly, Golden-Gate assembly, restriction enzyme digestion and ligation.
  • Experience with viral vectors (e.g., AAV/lentiviral).
  • Experience in single cell isolation and cloning and fluorescence-based cell identification using either microscopy or flow cytometry methods.
  • Experience in working with cGMP compliant/quality-controlled procedures is a plus.

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