Scientist, Molecular Biology/Expression (Antibody Engineering)

Company Description:

Molecular Templates is a clinical-stage biopharmaceutical company focused on the discovery and development of differentiated, targeted, biologic therapeutics. Our proprietary biologic drug platform technology, known as engineered toxin bodies, or ETBs, leverages the resident biology of a genetically engineered form of Shiga-like Toxin A subunit to create novel therapies with potent and differentiated mechanisms of action for cancer and other serious diseases. MTEM currently has multiple clinical-stage ETB candidates in development including MT-5111, TAK-169, and MT-6402. Additional preclinical compounds derived from our ETB platform technology are being advanced toward clinical development. Additional information about Molecular Templates can be obtained at www.mtem.com.

Position Overview:

MTEM is seeking a highly motivated individual to help build an antibody discovery group. This hands-on position involves generation and screening of antibodies to be used in the company's ETB scaffold for the development of novel therapeutics. This position will join a team in support of therapeutic antibody discovery programs. The ideal candidate will have hands on experience in plate-based PCR amplification of antibody variable regions and expression in both bacterial and mammalian systems.

Job Responsibilities:

• Design and execute strategies to amplify and clone antibody variable regions from both B-cell and phage display libraries.
• Optimization of primers and DNA templates.
• Plate-based bacterial transduction and mammalian transfection, expression and purification.

• Perform antibody purification in support of Screening technologies team
• Support high throughput screening of phage panning and Bcell outputs.
• Analyze antibody sequence data (Sanger and/or NGS)
• Maintain Laboratory stocks and reagents and serve as a mentor to junior staff
• Keeps a detailed and well-organized electronic laboratory notebook
• Presents data at group meetings
• Stays current with literature and emerging trends in the discipline

Qualifications:

• PhD in Molecular Biology, Microbiology, Biochemistry or related field with 2+ years of experience in an antibody discovery environment or
• Master's Degree and a minimum of 4 years of experience in an antibody discovery team in the pharmaceutical or biotechnology industry.
• Demonstrated experience in amplifying, cloning and expressing antibodies.
• Working knowledge of high-throughput plate-based processes.
• Expertise in molecular biology techniques (PCR, cloning, primer design, plasmid preparation, etc.).
• Experience with plate-based expression in bacterial and mammalian platforms.
• Use of DNA sequence analysis/editing using tools such as Geneious Biologics or Prime.
• Handling and manipulation of bacterial cultures.
• Antibody purification techniques and characterization.
• Experience with B cell cloning and Phage display.
• Familiarity with formatting scFV and vHH/nanobodies
• Ability to work on multiple projects in parallel to deliver results in rapid timeframes
• Ability to work independently in a dynamic environment
• Critical thinking, prioritization of tasks, attention to detail and troubleshooting
• Creativity in approaches to complex problems and desire to expand technical skillset
• Timely delivery of team goals and ability to build productive relationships with colleagues across MTEM's R&D department

Reporting Structure:

This position currently has no supervisory responsibilities. This position reports to the Vice President, Antibody Discovery.

MolecularTemplatescelebrates and supports our differences and is proud to be an Equal Employment Opportunity employer. We do not discriminate based upon race, religion, color, national origin,sex(including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, gender expression, age, veteran status, disability, or any other legally protected characteristic.

MTEM is committed toprovidingreasonable accommodations for qualifiedindividuals.Ifyou needassistancewith your applicationdue to a disability, you may contact us at:careers@mtem.com.

By submitting a resume for consideration, applicants verify that they have readMTEM's Applicant Privacy notice.https://www.mtem.com/privacy-policy