Associate Molecular Biologist

3 days left

Employer
Alpha Consulting
Location
La Jolla, California, US
Salary
Competitive
Posted
Jun 04, 2021
Closes
Jun 22, 2021
Ref
2210972743
Discipline
GIS
Employment Type
Full time
Salary Type
Salary
ASSOCIATE MOLECULAR BIOLOGIST
SAN DIEGO, CA

Project Description:
The Oncology Discovery team at the Oncogenesis Thematic Research Center is seeking a Research Associate to support our drug discovery and characterization efforts. The qualified candidate will leverage various CRISPR/Cas9 technologies to enable genetic screens to support early and late stage drug mechanism of action studies. They will assist senior team members in performing the totality of the screening process, from cell line generation to NGS library preparation and target validation. Common skills and techniques used will include mammalian cell culture, PCR, and western blot.
Responsibilities will include, but are not limited to, the following:
  • Design research protocols in collaboration with senior researchers
  • Execute existing cellular and molecular biology protocols in the laboratory.
  • Maintain cancer cell lines and establish new stable lines via lentiviral transduction.
  • Perform genetic targeting using siRNA, shRNA or CRISPR/Cas9 technology for cell line characterization and screening, including studies performed on a genome-wide scale.
  • Conduct routine molecular and cellular experiments including gDNA and RNA isolation, PCR, cloning, western blot analysis, flow cytometry, and microplate cell proliferation assays.
  • Identify experimental issues, troubleshoot, and recommend a corrective course of action.
  • Record data in electronic notebook in accordance to company policy
  • Communicate results in written and oral presentations.

Required Skills:
Required Competencies: Essential Knowledge, Skills, & Abilities:
  • Bachelor's degree in a scientific discipline (no PhD candidates)
  • 1-2 years pre- or post-undergraduate, wet lab research experience required.
  • 1-2 years of wet lab research experience and basic knowledge of cancer biology
  • Understanding of mammalian cell culture and sterile technique
  • Ability to multi-task and prioritize time management of daily activities to deliver timely results.
  • Excellent oral and written communication skills
  • Capable of working collaboratively in a multidisciplinary environment.
  • Basic molecular biology skills, including PCR and western blotting, highly preferred.
Additional Job Requirements:
  • Position Handles Hazardous Materials

This 12+ month position starts ASAP.

Please E-MAIL your resume (attachment to email) with rate and availability to Karen: karen@alphaconsulting.com

ALPHA'S REQUIREMENT #21-01773
W2 ONLY MUST BE ELIGIBLE TO WORK IN THE U.S. AS AN HOURLY W2 EMPLOYEE
#ZR
#LI-KG1

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