Scientist I - Cell Biology R&D

4 days left

Employer
Thermo Fisher Scientific
Location
Carlsbad, California, US
Salary
Competitive
Posted
Jun 04, 2021
Closes
Jun 16, 2021
Ref
2216620582
Discipline
Biology
Employment Type
Full time
Salary Type
Salary
When you're part of the team at Thermo Fisher Scientific, you'll do important work, like helping customers in finding cures for cancer, protecting the environment or making sure our food is safe. Your work will have real-world impact, and you'll be supported in achieving your career goals.
Location/Division Specific Information
Carlsbad, CA | Biosciences Division
How will you make an impact?
Be part of the genome engineering revolution. This scientist will develop tools and techniques to support genome engineering. This scientist will be expected to work in a highly collaborative team environment, supporting some projects with some supervision of a senior scientist and leading some projects independently. Projects may include, but not be limited to, cloning, developing gene editing strategies, and developing assays to monitor editing.
What will you do?
- Molecular biology: plasmid preparation, DNA/RNA extraction, subcloning, PCR and gene synthesis.
- Sanger and NGS (Ion-Torrent or illumine platform) sequencing: library sample preparation, data process and analysis
- Genome editing: CRISPR/Cas9 in vitro and in cell editing. On-target, HDR and off-target assay
- In vitro transcription: RNA and ssDNA synthesis
- Protein purification: HPLC and other column purification skill.
- Mammalian cell culture and flow cytometry assay: especially in T cell and iPSC.
- Data analysis: using excel, vector analysis tool, sequencing analysis tool
How will you get here?
Education
- A bachelor's degree with 2+ years of experience in cell biology
- A master's degree with 1+ years of experience in cell biology preferred
Experience
- Good cloning experience highly preferred. Plasmid constructs, DNA/gene synthesis, cloning and amplification
- Genome editing using CRISPR/Cas9
- Primary cell culture, transfection, and flow cytometry (CAR T experience preferred)
- NGS (Ion-Torrent or Illumina) based sample preparation and analysis experience preferred
Knowledge, Skills, Abilities
- Data analysis, troubleshooting and suggesting alternatives
- Design and plan experiments independently
- Strong teamwork orientation with demonstrable influence/interpersonal skills
- Maintain a detailed lab notebook and present your findings in a group setting
Thermo Fisher Scientific is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, creed, religion, color, national or ethnic origin, citizenship, sex, sexual orientation, gender identity and expression, genetic information, veteran status, age or disability status.

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