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Biologist (Aseptic Technique and Mammalian Cell Culture)

Employer
GAP Solutions Inc
Location
Gaithersburg, Maryland, US
Salary
Competitive
Closing date
May 16, 2021

View more

Sector
Academic / Research, Consultancy/Private Sector
Field
Conservation science
Discipline
Other
Salary Type
Salary
Employment Type
Full time
Position Objective: Provide services as a Biologist in support of the overall functions of the Vaccine Research Center (VRC), Vaccine Production Program (VPP) within the National Institute of Allergy and Infectious Diseases (NIAID).
Duties and Responsibilities:
- Contribute to the generation of stable cell lines for vaccine and therapeutic protein candidates including monoclonal antibodies, recombinant proteins, and virus-like particles.
- Independently execute each step of the cell line development process including plasmid production, transfection, single cell cloning, clone expansion in static culture plates and shake flasks, fed-batch analysis, and clone stability studies.
- Use automated platforms for clone selection and screening.
- Prepare plasmid DNA using standard molecular cloning techniques.
- Participate in cell line development platform design, optimization, and cycle-time reduction studies including methods for clone selection, expansion, ranking, and characterization.
- Perform small-scale process development studies using various culture vessels including tissue culture plates, shake flasks, and small-scale bioreactors.
- Generate stable pools and perform transient protein production for supply of reagent-grade material.
- Provide laboratory support for medium preparation, equipment maintenance, and ordering of supplies.
- Write and review technical reports documenting process development studies and stable cell line generation work.
- Work with other team members to generate experimental plans for process development studies.
- Write and review standard operating procedures for cell line development processes and instrumentation.
- Maintain complete documentation records, including a detailed and up-to-date laboratory notebook, to support regulatory filings.
- Regularly communicate progress to team lead and present work to cell line development team.
Basic Qualifications:
- B.S. in Biological Sciences with a minimum of 3 years of relevant work experience or M.S. in Biological Sciences with independent research experience in an academic or industrial setting
- Hands-on experience with aseptic technique and mammalian cell culture along with supporting technologies for cell line engineering including cell counting, high-throughput transfection, single-cell cloning, and flow cytometry
- Familiarity with molecular cloning methods, PCR, and agarose gel electrophoresis
- Experience in preparing technical reports, standard operating procedures, and data presentations
Minimum Qualifications:
- Experience in monoclonal antibody and/or vaccine production preferred
- Ability to work independently to execute defined experiments and troubleshoot issues as needed to meet project timelines
- Ability to effectively communicate plans, results, challenges, and ideas to other team members in oral and written forms
- Detail-oriented and possess strong organizational skills with the ability to prioritize multiple tasks and projects
To perform this job successfully, an individual must be able to perform each essential duty satisfactorily. The requirements listed above are representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.
*This job description is not designed to cover or contain a comprehensive listing of activities, duties or responsibilities that are required by this position.
To perform this job successfully, an individual must be able to perform each essential duty satisfactorily. The requirements listed above are representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.
GAP Solutions provides reasonable accommodations to qualified individuals with disabilities. If you need an accommodation to apply for a job us at recruiting@gapsi.com . You will need to reference the requisition number of the position in which you are interested. Your message will be routed to the appropriate recruiter who will assist you. Please note, this email address is only to be used for those individuals who need an accommodation to apply for a job. Emails for any other reason or those that do not include a requisition number will not be returned.
GAP Solutions is an Equal Opportunity/Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to their race, ethnicity, ancestry, color, sex, religion, creed, age, national origin, citizenship status, disability, medical condition, military and veteran status, marital status, sexual orientation or perceived sexual orientation, gender, gender identity, and gender expression, familial status, political affiliation, genetic information, or any other legally protected status or characteristics.
This position is contingent upon contract award.
Requisition ID: 4193

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