Scientist I - Molecular Biology and Microbial Strain Engineering

Employer
Sanofi
Location
La Jolla, California, US
Salary
Competitive
Posted
May 04, 2021
Closes
May 08, 2021
Ref
2160166475
Discipline
Biology, Genetics
Employment Type
Full time
Salary Type
Salary
Scientist I - Molecular Biology and Microbial Strain Engineering Synthorx, a Sanofi Company, is a biopharmaceuticals company dedicated to delivering new biologic medicines to patients. Synthorx has developed a revolutionary technology based on bacterial cells that utilize a six-letter, semi-synthetic DNA code to produce custom proteins with site-specific functionalities for the discovery and development of novel protein-based therapeutics. We are seeking a creative and experienced molecular biologist to join our team to continue development of our technology and apply it to the development of new drug candidates. The ideal candidate will bring extensive experience in molecular biology, DNA assembly, and protein engineering. More information can be found on our website: www.synthorx.com To apply for this position, applicants are encouraged to include a resume and cover letter describing your experience, interests, and any other information that you would consider interesting and relevant. Position Overview: This position reports to Synthorx's Head of Platform and Strain development and will leverage synthetic biology to drive further development and application of Synthorx's proprietary E. coli expression platform. The ideal candidate will be a creative and innovative problem-solver with strong attention to detail, have strong communication skills, and the ability to work effectively within a highly-collaborative environment with other team members and other groups at the company. Primary Responsibilities:
  • Design and clone DNA constructs, and apply Synthorx's expanded DNA alphabet technology, using modern molecular biology techniques, such as Golden Gate Assembly, Gibson Assembly, etc.
  • Perform genomic modifications in E. coli using various methods and techniques such as Lambda Red recombineering, CRISPR, MAGE, etc.
  • Design, perform, and analyze recombinant protein expression experiments to evaluate constructs and engineered strains, and apply findings to further improve protein expression and other strain parameters
  • Integrate feedback and results from other research groups (fermentation, protein purification, analytics, and pharmacology) to assess and design improved constructs and strains
  • Develop innovative screening and/or selection methods to improve recombinant protein expression and strain performance, including the development of model expression systems at various scales
  • Innovate improvements to the team's design-build-test-learn workflow via methodological improvements and new instrumentation
  • Write associated reports, maintain laboratory notebooks, and present findings to other team members and groups at the company
Education Requirements:
  • Ph.D. (molecular biology, bioengineering, microbiology, synthetic biology or a closely related field) or equivalent experience, and at least 2 years post-graduate work as a scientist in industry or academia; exceptional candidates without post-doctorate experience will be considered.
Basic Qualifications:
  • Proficiency and extensive experience with modern molecular biology techniques including DNA construct design, PCR, DNA assembly and cloning, plasmid isolation, and DNA sequencing, as well as with associated software for construct design and sequencing analysis (Snapgene, Geneious, etc.)
  • Experience with and practical knowledge of bacterial genetics, microbiology, and synthetic biology, particularly strain modification and engineering
  • Experience and expertise with protein engineering and recombinant protein expression, preferably in E. coli
  • Experience with DNA library construction methodologies and developing/executing directed evolution experiments
  • Highly self-motivated and independent, but demonstrated ability to coordinate and work well as part of a research team
  • Ability to work under pressure to meet deadlines and excellent multitasking skills
  • Strong organizational and communication skills are imperative
Additional experience and skills not required, but helpful:
  • NGS data analysis and bioinformatics
  • SPR or other protein-protein interaction measurement methodologies
  • E. coli fermentation
The Company provides competitive salaries and benefits. At Sanofi diversity and inclusion is foundational to how we operate and embedded in our Core Values. We recognize to truly tap into the richness diversity brings we must lead with inclusion and have a workplace where those differences can thrive and be leveraged to empower the lives of our colleagues, patients and customers. We respect and celebrate the diversity of our people, their backgrounds and experiences and provide equal opportunity for all. At Sanofi diversity and inclusion is foundational to how we operate and embedded in our Core Values. We recognize to truly tap into the richness diversity brings we must lead with inclusion and have a workplace where those differences can thrive and be leveraged to empower the lives of our colleagues, patients and customers. We respect and celebrate the diversity of our people, their backgrounds and experiences and provide equal opportunity for all.
Sanofi Inc. and its U.S. affiliates are Equal Opportunity and Affirmative Action employers committed to a culturally diverse workforce. All qualified applicants will receive consideration for employment without regard to race; color; creed; religion; national origin; age; ancestry; nationality; marital, domestic partnership or civil union status; sex, gender, gender identity or expression; affectional or sexual orientation; disability; veteran or military status or liability for military status; domestic violence victim status; atypical cellular or blood trait; genetic information (including the refusal to submit to genetic testing) or any other characteristic protected by law. #GD-SA
#LI-SA

At Sanofi diversity and inclusion is foundational to how we operate and embedded in our Core Values. We recognize to truly tap into the richness diversity brings we must lead with inclusion and have a workplace where those differences can thrive and be leveraged to empower the lives of our colleagues, patients and customers. We respect and celebrate the diversity of our people, their backgrounds and experiences and provide equal opportunity for all.

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