Biologist (5-7 years)

Title: Biologist

Location: San Diego, CA

Start date: ASAP

Responsibilities:

• Protein purification and analytics work, including the hands-on execution of purification and/or characterization of protein reagents for structural biology and biochemical assays. Proficiency in column chromatography for proteins, protein concentration, and protein analytical techniques will be key to this position.
• High-throughput purification of recombinant proteins for crystallization studies and biochemical assays using standard chromatographic methods and automated parallel processing systems
• Lysis of prokaryotic and eukaryotic cell pellets and clarification of lysates by centrifugation
• Protein purification by affinity, ion exchange, and size exclusion chromatography
• Analysis of protein purity using SDS-page gel electrophoresis
• Quantitation of protein concentration by Bradford assay and UV spectroscopy
• Accurate and timely entry of all experimental data into a Laboratory Information Management System (LIMS) and Laboratory notebooks
• Storage, retrieval, and shipping of protein samples utilizing Microsoft Excel, barcodes, and LIMS
• Interface between Purification, Mass Spectrometry, and Crystallization groups regarding protein samples
• Interact effectively with other team members to address technical challenges

Requirements:

• Must have a Bachelor's Degree in Biological Sciences (or related field) and minimum of 5 years laboratory experience in either industry or academia
• Must have experience with standard protein purification techniques including affinity, ion exchange, and size exclusion chromatography; analysis of protein purity by SDS-page gel electrophoresis; and determination of protein concentration by Bradford assay and OD 280nm.
• Familiar with AKTA PURE and related protein chromatography instruments
• Must exhibit the ability to solve problems, be self-motivated and capable of working independently, while contributing to a project team environment
• The candidate must be highly organized and effective at multi-tasking, with the ability to run several purification experiments simultaneously
• Attention to detail and ability to keep accurate records
• Conscientious, with a strong work ethic
• Adherence to ‘Good Research Practices'
• Good written and oral communication skills and strong computer skills are essential

Joule, a System One division is a leader in specialized workforce solutions and integrated services. With more than 6,000 employees and roughly 50 offices throughout the U.S. we provide scientific, clinical, engineering, energy, IT, legal and administrative staffing services. For more than 40 years, we have delivered workforce solutions and integrated services to clients nationally.